Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 74531056
Single-end alignments with a unique best hit 21379604
Sequences without alignments under any condition 22542289
Sequences that did not map uniquely 30609163
Genomic sequence context not extractable (edges of chromosomes) 23016

Cytosine Methylation

Total C's analysed 134402420
Methylated C's in CpG context 22135048
Methylated C's in CHG context 633998
Methylated C's in CHH context 7135489
Methylated C's in Unknown context 4243
Unmethylated C's in CpG context 3717836
Unmethylated C's in CHG context 26058014
Unmethylated C's in CHH context 74722035
Unmethylated C's in Unknown context 26107
Percentage methylation (CpG context) 85.6%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4899455 original top strand
CTOT 5819536 complementary to original top strand
CTOB 5772152 complementary to original bottom strand
OB 4865445 original bottom strand

Deduplication

Alignments analysed 21356588
Unique alignments 11923785
Duplicates removed 9432803
Duplicated alignments were found at 2735889 different positions

Cytosine Methylation after Extraction

Total C's analysed 73264966
Methylated C's in CpG context 10020093
Methylated C's in CHG context 387586
Methylated C's in CHH context 5124999
Unmethylated C's in CpG context 2096343
Unmethylated C's in CHG context 13149191
Unmethylated C's in CHH context 42486754
Percentage methylation (CpG context) 82.7%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 10.8%

M-Bias Plot