Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 67984205
Single-end alignments with a unique best hit 20005064
Sequences without alignments under any condition 20315504
Sequences that did not map uniquely 27663637
Genomic sequence context not extractable (edges of chromosomes) 20159

Cytosine Methylation

Total C's analysed 126658476
Methylated C's in CpG context 21430002
Methylated C's in CHG context 605325
Methylated C's in CHH context 6679570
Methylated C's in Unknown context 4040
Unmethylated C's in CpG context 3061995
Unmethylated C's in CHG context 25424126
Unmethylated C's in CHH context 69457458
Unmethylated C's in Unknown context 25389
Percentage methylation (CpG context) 87.5%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4505105 original top strand
CTOT 5524316 complementary to original top strand
CTOB 5475061 complementary to original bottom strand
OB 4480423 original bottom strand

Deduplication

Alignments analysed 19984905
Unique alignments 10802610
Duplicates removed 9182295
Duplicated alignments were found at 2586577 different positions

Cytosine Methylation after Extraction

Total C's analysed 66776329
Methylated C's in CpG context 9440679
Methylated C's in CHG context 363316
Methylated C's in CHH context 4683749
Unmethylated C's in CpG context 1675246
Unmethylated C's in CHG context 12344351
Unmethylated C's in CHH context 38268988
Percentage methylation (CpG context) 84.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 10.9%

M-Bias Plot