Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 87834637 Number of alignments with a unique best hit from the different alignments: 25540537 Mapping efficiency: 29.1% Sequences with no alignments under any condition: 26159848 Sequences did not map uniquely: 36134252 Sequences which were discarded because genomic sequence could not be extracted: 24870 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 5727222 ((converted) top strand) CT/GA: 5701238 ((converted) bottom strand) GA/CT: 7068691 (complementary to (converted) top strand) GA/GA: 7018516 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 158553310 Total methylated C's in CpG context: 24149603 Total methylated C's in CHG context: 702690 Total methylated C's in CHH context: 8156302 Total methylated C's in Unknown context: 4326 Total unmethylated C's in CpG context: 4183914 Total unmethylated C's in CHG context: 30112585 Total unmethylated C's in CHH context: 91248216 Total unmethylated C's in Unknown context: 30706 C methylated in CpG context: 85.2% C methylated in CHG context: 2.3% C methylated in CHH context: 8.2% C methylated in Unknown context (CN or CHN): 12.3% Bismark completed in 0d 1h 51m 35s