Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 87834637
Single-end alignments with a unique best hit 25540537
Sequences without alignments under any condition 26159848
Sequences that did not map uniquely 36134252
Genomic sequence context not extractable (edges of chromosomes) 24870

Cytosine Methylation

Total C's analysed 158553310
Methylated C's in CpG context 24149603
Methylated C's in CHG context 702690
Methylated C's in CHH context 8156302
Methylated C's in Unknown context 4326
Unmethylated C's in CpG context 4183914
Unmethylated C's in CHG context 30112585
Unmethylated C's in CHH context 91248216
Unmethylated C's in Unknown context 30706
Percentage methylation (CpG context) 85.2%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5727222 original top strand
CTOT 7068691 complementary to original top strand
CTOB 7018516 complementary to original bottom strand
OB 5701238 original bottom strand

Deduplication

Alignments analysed 25515667
Unique alignments 14431531
Duplicates removed 11084136
Duplicated alignments were found at 3322076 different positions

Cytosine Methylation after Extraction

Total C's analysed 87759971
Methylated C's in CpG context 11235176
Methylated C's in CHG context 442616
Methylated C's in CHH context 5958507
Unmethylated C's in CpG context 2398111
Unmethylated C's in CHG context 15471847
Unmethylated C's in CHH context 52253714
Percentage methylation (CpG context) 82.4%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 10.2%

M-Bias Plot