Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 64352550 Number of alignments with a unique best hit from the different alignments: 19721209 Mapping efficiency: 30.6% Sequences with no alignments under any condition: 18442450 Sequences did not map uniquely: 26188891 Sequences which were discarded because genomic sequence could not be extracted: 20168 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4410505 ((converted) top strand) CT/GA: 4370600 ((converted) bottom strand) GA/CT: 5484879 (complementary to (converted) top strand) GA/GA: 5435057 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 123608897 Total methylated C's in CpG context: 19125117 Total methylated C's in CHG context: 556295 Total methylated C's in CHH context: 6410972 Total methylated C's in Unknown context: 3425 Total unmethylated C's in CpG context: 3215880 Total unmethylated C's in CHG context: 24861808 Total unmethylated C's in CHH context: 69438825 Total unmethylated C's in Unknown context: 22722 C methylated in CpG context: 85.6% C methylated in CHG context: 2.2% C methylated in CHH context: 8.5% C methylated in Unknown context (CN or CHN): 13.1% Bismark completed in 0d 1h 23m 45s