Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 59459431 Number of alignments with a unique best hit from the different alignments: 20145696 Mapping efficiency: 33.9% Sequences with no alignments under any condition: 17101979 Sequences did not map uniquely: 22211756 Sequences which were discarded because genomic sequence could not be extracted: 16457 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4509629 ((converted) top strand) CT/GA: 4475635 ((converted) bottom strand) GA/CT: 5594115 (complementary to (converted) top strand) GA/GA: 5549860 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 120343086 Total methylated C's in CpG context: 13832027 Total methylated C's in CHG context: 461081 Total methylated C's in CHH context: 6664085 Total methylated C's in Unknown context: 2655 Total unmethylated C's in CpG context: 3413355 Total unmethylated C's in CHG context: 24010493 Total unmethylated C's in CHH context: 71962045 Total unmethylated C's in Unknown context: 19915 C methylated in CpG context: 80.2% C methylated in CHG context: 1.9% C methylated in CHH context: 8.5% C methylated in Unknown context (CN or CHN): 11.8% Bismark completed in 0d 1h 20m 47s