Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 20145696
Sequences without alignments under any condition 17101979
Sequences that did not map uniquely 22211756
Genomic sequence context not extractable (edges of chromosomes) 16457

Cytosine Methylation

Total C's analysed 120343086
Methylated C's in CpG context 13832027
Methylated C's in CHG context 461081
Methylated C's in CHH context 6664085
Methylated C's in Unknown context 2655
Unmethylated C's in CpG context 3413355
Unmethylated C's in CHG context 24010493
Unmethylated C's in CHH context 71962045
Unmethylated C's in Unknown context 19915
Percentage methylation (CpG context) 80.2%
Percentage methylation (CHG context) 1.9%
Percentage methylation (CHH context) 8.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4509629 original top strand
CTOT 5594115 complementary to original top strand
CTOB 5549860 complementary to original bottom strand
OB 4475635 original bottom strand

Deduplication

Alignments analysed 20129239
Unique alignments 13660276
Duplicates removed 6468963
Duplicated alignments were found at 2609514 different positions

Cytosine Methylation after Extraction

Total C's analysed 80756458
Methylated C's in CpG context 8385192
Methylated C's in CHG context 335216
Methylated C's in CHH context 5285129
Unmethylated C's in CpG context 2342276
Unmethylated C's in CHG context 15323134
Unmethylated C's in CHH context 49085511
Percentage methylation (CpG context) 78.2%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 9.7%

M-Bias Plot