Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_11_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 54458572
Single-end alignments with a unique best hit 15680143
Sequences without alignments under any condition 16326025
Sequences that did not map uniquely 22452404
Genomic sequence context not extractable (edges of chromosomes) 18576

Cytosine Methylation

Total C's analysed 100296882
Methylated C's in CpG context 17662617
Methylated C's in CHG context 491939
Methylated C's in CHH context 5196171
Methylated C's in Unknown context 3246
Unmethylated C's in CpG context 2900560
Unmethylated C's in CHG context 19823190
Unmethylated C's in CHH context 54222405
Unmethylated C's in Unknown context 19966
Percentage methylation (CpG context) 85.9%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3528573 original top strand
CTOT 4336953 complementary to original top strand
CTOB 4298059 complementary to original bottom strand
OB 3497982 original bottom strand

Deduplication

Alignments analysed 15661567
Unique alignments 8177897
Duplicates removed 7483670
Duplicated alignments were found at 1903267 different positions

Cytosine Methylation after Extraction

Total C's analysed 50769500
Methylated C's in CpG context 7110525
Methylated C's in CHG context 280561
Methylated C's in CHH context 3592530
Unmethylated C's in CpG context 1619619
Unmethylated C's in CHG context 9193433
Unmethylated C's in CHH context 28972832
Percentage methylation (CpG context) 81.4%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 11.0%

M-Bias Plot