Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_10_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 78221457
Single-end alignments with a unique best hit 25462569
Sequences without alignments under any condition 21496817
Sequences that did not map uniquely 31262071
Genomic sequence context not extractable (edges of chromosomes) 30186

Cytosine Methylation

Total C's analysed 159765962
Methylated C's in CpG context 24639901
Methylated C's in CHG context 738394
Methylated C's in CHH context 8517097
Methylated C's in Unknown context 4229
Unmethylated C's in CpG context 4431826
Unmethylated C's in CHG context 33913328
Unmethylated C's in CHH context 87525416
Unmethylated C's in Unknown context 27106
Percentage methylation (CpG context) 84.8%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 8.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6528162 original top strand
CTOT 6260193 complementary to original top strand
CTOB 6190688 complementary to original bottom strand
OB 6453340 original bottom strand

Deduplication

Alignments analysed 25432383
Unique alignments 8934789
Duplicates removed 16497594
Duplicated alignments were found at 3882578 different positions

Cytosine Methylation after Extraction

Total C's analysed 54706781
Methylated C's in CpG context 6634857
Methylated C's in CHG context 291620
Methylated C's in CHH context 4272996
Unmethylated C's in CpG context 1559899
Unmethylated C's in CHG context 10265526
Unmethylated C's in CHH context 31681883
Percentage methylation (CpG context) 81.0%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 11.9%

M-Bias Plot