Bismark report for: /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F2_S22_L002_R1_001_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F2_S22_L002_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/caligus/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 81606018 Number of paired-end alignments with a unique best hit: 17584142 Mapping efficiency: 21.5% Sequence pairs with no alignments under any condition: 51170344 Sequence pairs did not map uniquely: 12851532 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4441844 ((converted) top strand) GA/CT/CT: 4370400 (complementary to (converted) top strand) GA/CT/GA: 4356733 (complementary to (converted) bottom strand) CT/GA/GA: 4415163 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 761182841 Total methylated C's in CpG context: 1619428 Total methylated C's in CHG context: 1312509 Total methylated C's in CHH context: 7923134 Total methylated C's in Unknown context: 866500 Total unmethylated C's in CpG context: 122527945 Total unmethylated C's in CHG context: 127216928 Total unmethylated C's in CHH context: 500582897 Total unmethylated C's in Unknown context: 6668909 C methylated in CpG context: 1.3% C methylated in CHG context: 1.0% C methylated in CHH context: 1.6% C methylated in unknown context (CN or CHN): 11.5% Bismark completed in 0d 4h 19m 9s