Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F2_S22_L001_R1_001_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F2_S22_L001_R2_001_val_2.fq.gz

Data processed at 15:57 on 2020-02-04


Alignment Stats

Sequence pairs analysed in total 82079568
Paired-end alignments with a unique best hit 17899898
Pairs without alignments under any condition 51138153
Pairs that did not map uniquely 13041517
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 776913879
Methylated C's in CpG context 1643234
Methylated C's in CHG context 1332444
Methylated C's in CHH context 8089457
Methylated C's in Unknown context 887874
Unmethylated C's in CpG context 125034032
Unmethylated C's in CHG context 129772483
Unmethylated C's in CHH context 511042229
Unmethylated C's in Unknown context 6849496
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4522150 original top strand
CTOT 4446081 complementary to original top strand
CTOB 4433178 complementary to original bottom strand
OB 4498489 original bottom strand

Deduplication

Alignments analysed 17899898
Unique alignments 14474459
Duplicates removed 3425439
Duplicated alignments were found at 2305947 different positions

Cytosine Methylation after Extraction

Total C's analysed 370895321
Methylated C's in CpG context 729489
Methylated C's in CHG context 635519
Methylated C's in CHH context 3932393
Unmethylated C's in CpG context 58931092
Unmethylated C's in CHG context 61055902
Unmethylated C's in CHH context 245610926
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.6%

M-Bias Plot