Bismark report for: /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F1_S20_L002_R1_001_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F1_S20_L002_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/caligus/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 112493002 Number of paired-end alignments with a unique best hit: 31358065 Mapping efficiency: 27.9% Sequence pairs with no alignments under any condition: 60671476 Sequence pairs did not map uniquely: 20463461 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8897914 ((converted) top strand) GA/CT/CT: 6751598 (complementary to (converted) top strand) GA/CT/GA: 6786829 (complementary to (converted) bottom strand) CT/GA/GA: 8921722 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1343425791 Total methylated C's in CpG context: 2886711 Total methylated C's in CHG context: 2595053 Total methylated C's in CHH context: 14140740 Total methylated C's in Unknown context: 1548914 Total unmethylated C's in CpG context: 204897578 Total unmethylated C's in CHG context: 214379403 Total unmethylated C's in CHH context: 904526306 Total unmethylated C's in Unknown context: 12233657 C methylated in CpG context: 1.4% C methylated in CHG context: 1.2% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 11.2% Bismark completed in 0d 6h 51m 14s