Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F1_S20_L002_R1_001_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F1_S20_L002_R2_001_val_2.fq.gz

Data processed at 15:57 on 2020-02-04


Alignment Stats

Sequence pairs analysed in total 112493002
Paired-end alignments with a unique best hit 31358065
Pairs without alignments under any condition 60671476
Pairs that did not map uniquely 20463461
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 1343425791
Methylated C's in CpG context 2886711
Methylated C's in CHG context 2595053
Methylated C's in CHH context 14140740
Methylated C's in Unknown context 1548914
Unmethylated C's in CpG context 204897578
Unmethylated C's in CHG context 214379403
Unmethylated C's in CHH context 904526306
Unmethylated C's in Unknown context 12233657
Percentage methylation (CpG context) 1.4%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8897914 original top strand
CTOT 6751598 complementary to original top strand
CTOB 6786829 complementary to original bottom strand
OB 8921722 original bottom strand

Deduplication

Alignments analysed 31358063
Unique alignments 23092109
Duplicates removed 8265954
Duplicated alignments were found at 5371098 different positions

Cytosine Methylation after Extraction

Total C's analysed 614541903
Methylated C's in CpG context 1242538
Methylated C's in CHG context 1173030
Methylated C's in CHH context 6425721
Unmethylated C's in CpG context 92014349
Unmethylated C's in CHG context 96183801
Unmethylated C's in CHH context 417502464
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.5%

M-Bias Plot