Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F1_S20_L001_R1_001_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F1_S20_L001_R2_001_val_2.fq.gz

Data processed at 15:57 on 2020-02-04


Alignment Stats

Sequence pairs analysed in total 113253920
Paired-end alignments with a unique best hit 31988553
Pairs without alignments under any condition 60463531
Pairs that did not map uniquely 20801836
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 1374241887
Methylated C's in CpG context 2947829
Methylated C's in CHG context 2642920
Methylated C's in CHH context 14444000
Methylated C's in Unknown context 1591086
Unmethylated C's in CpG context 209503774
Unmethylated C's in CHG context 219185230
Unmethylated C's in CHH context 925518134
Unmethylated C's in Unknown context 12593816
Percentage methylation (CpG context) 1.4%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 9072203 original top strand
CTOT 6886524 complementary to original top strand
CTOB 6924159 complementary to original bottom strand
OB 9105666 original bottom strand

Deduplication

Alignments analysed 31988552
Unique alignments 23357515
Duplicates removed 8631037
Duplicated alignments were found at 5580519 different positions

Cytosine Methylation after Extraction

Total C's analysed 624176585
Methylated C's in CpG context 1261403
Methylated C's in CHG context 1188435
Methylated C's in CHH context 6524215
Unmethylated C's in CpG context 93398123
Unmethylated C's in CHG context 97616651
Unmethylated C's in CHH context 424187758
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.5%

M-Bias Plot