Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/oil/20150414_trimmed_2112_lane1_HB16_Oil_25000ppm_TTAGGC.fastq.gz

Data processed at 09:33:29 on 2022-02-07


Alignment Stats

Sequences analysed in total 21462840
Single-end alignments with a unique best hit 10790387
Sequences without alignments under any condition 5480515
Sequences that did not map uniquely 5191938
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 212797610
Methylated C's in CpG context 31770372
Methylated C's in CHG context 8211495
Methylated C's in CHH context 25943978
Methylated C's in Unknown context 310267
Unmethylated C's in CpG context 14009418
Unmethylated C's in CHG context 46372561
Unmethylated C's in CHH context 86489786
Unmethylated C's in Unknown context 682173
Percentage methylation (CpG context) 69.4%
Percentage methylation (CHG context) 15.0%
Percentage methylation (CHH context) 23.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 459450 original top strand
CTOT 4891423 complementary to original top strand
CTOB 4973472 complementary to original bottom strand
OB 466042 original bottom strand

Deduplication

Alignments analysed 10790387
Unique alignments 5189978
Duplicates removed 5600409
Duplicated alignments were found at 1680694 different positions

Cytosine Methylation after Extraction

Total C's analysed 100573067
Methylated C's in CpG context 12227583
Methylated C's in CHG context 5548341
Methylated C's in CHH context 18517042
Unmethylated C's in CpG context 7392033
Unmethylated C's in CHG context 18592605
Unmethylated C's in CHH context 38295463
Percentage methylation (CpG context) 62.3%
Percentage methylation (CHG context) 23.0%
Percentage methylation (CHH context) 32.6%

M-Bias Plot