Bismark report for: /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L002_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L002_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/caligus/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 81699034 Number of paired-end alignments with a unique best hit: 9286200 Mapping efficiency: 11.4% Sequence pairs with no alignments under any condition: 67407280 Sequence pairs did not map uniquely: 5005554 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2342924 ((converted) top strand) GA/CT/CT: 2317559 (complementary to (converted) top strand) GA/CT/GA: 2300451 (complementary to (converted) bottom strand) CT/GA/GA: 2325266 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 520231008 Total methylated C's in CpG context: 2514329 Total methylated C's in CHG context: 1950069 Total methylated C's in CHH context: 8457985 Total methylated C's in Unknown context: 1742408 Total unmethylated C's in CpG context: 82281700 Total unmethylated C's in CHG context: 85424357 Total unmethylated C's in CHH context: 339602568 Total unmethylated C's in Unknown context: 5203451 C methylated in CpG context: 3.0% C methylated in CHG context: 2.2% C methylated in CHH context: 2.4% C methylated in unknown context (CN or CHN): 25.1% Bismark completed in 0d 4h 21m 36s