Bismark report for: /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L001_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L001_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/caligus/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 82177525 Number of paired-end alignments with a unique best hit: 9562005 Mapping efficiency: 11.6% Sequence pairs with no alignments under any condition: 67475294 Sequence pairs did not map uniquely: 5140226 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2412884 ((converted) top strand) GA/CT/CT: 2384062 (complementary to (converted) top strand) GA/CT/GA: 2370078 (complementary to (converted) bottom strand) CT/GA/GA: 2394981 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 535876331 Total methylated C's in CpG context: 2593525 Total methylated C's in CHG context: 2007967 Total methylated C's in CHH context: 8733191 Total methylated C's in Unknown context: 1806572 Total unmethylated C's in CpG context: 84745811 Total unmethylated C's in CHG context: 87968326 Total unmethylated C's in CHH context: 349827511 Total unmethylated C's in Unknown context: 5392599 C methylated in CpG context: 3.0% C methylated in CHG context: 2.2% C methylated in CHH context: 2.4% C methylated in unknown context (CN or CHN): 25.1% Bismark completed in 0d 4h 24m 58s