Bismark report for: /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/caligus/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 112706276 Number of paired-end alignments with a unique best hit: 17528111 Mapping efficiency: 15.6% Sequence pairs with no alignments under any condition: 86586560 Sequence pairs did not map uniquely: 8591605 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4922482 ((converted) top strand) GA/CT/CT: 3813994 (complementary to (converted) top strand) GA/CT/GA: 3840498 (complementary to (converted) bottom strand) CT/GA/GA: 4951136 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 949354763 Total methylated C's in CpG context: 3989154 Total methylated C's in CHG context: 3288380 Total methylated C's in CHH context: 14679832 Total methylated C's in Unknown context: 2785517 Total unmethylated C's in CpG context: 141676841 Total unmethylated C's in CHG context: 148657726 Total unmethylated C's in CHH context: 637062830 Total unmethylated C's in Unknown context: 9671815 C methylated in CpG context: 2.7% C methylated in CHG context: 2.2% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 22.4% Bismark completed in 0d 6h 50m 20s