Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R2_001.fastq.gz

Data processed at 11:17 on 2020-01-31


Alignment Stats

Sequence pairs analysed in total 112706276
Paired-end alignments with a unique best hit 17528111
Pairs without alignments under any condition 86586560
Pairs that did not map uniquely 8591605
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 949354763
Methylated C's in CpG context 3989154
Methylated C's in CHG context 3288380
Methylated C's in CHH context 14679832
Methylated C's in Unknown context 2785517
Unmethylated C's in CpG context 141676841
Unmethylated C's in CHG context 148657726
Unmethylated C's in CHH context 637062830
Unmethylated C's in Unknown context 9671815
Percentage methylation (CpG context) 2.7%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4922482 original top strand
CTOT 3813994 complementary to original top strand
CTOB 3840498 complementary to original bottom strand
OB 4951136 original bottom strand

Deduplication

Alignments analysed 17528110
Unique alignments 13590786
Duplicates removed 3937324
Duplicated alignments were found at 2738599 different positions

Cytosine Methylation after Extraction

Total C's analysed 480985368
Methylated C's in CpG context 1723628
Methylated C's in CHG context 1666356
Methylated C's in CHH context 7384205
Unmethylated C's in CpG context 71093153
Unmethylated C's in CHG context 74575038
Unmethylated C's in CHH context 324542988
Percentage methylation (CpG context) 2.4%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.2%

M-Bias Plot