Bismark report for: /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L001_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L001_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/caligus/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 113474961 Number of paired-end alignments with a unique best hit: 18071283 Mapping efficiency: 15.9% Sequence pairs with no alignments under any condition: 86568022 Sequence pairs did not map uniquely: 8835656 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5072942 ((converted) top strand) GA/CT/CT: 3933628 (complementary to (converted) top strand) GA/CT/GA: 3960012 (complementary to (converted) bottom strand) CT/GA/GA: 5104700 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 979199135 Total methylated C's in CpG context: 4118192 Total methylated C's in CHG context: 3393802 Total methylated C's in CHH context: 15178693 Total methylated C's in Unknown context: 2887891 Total unmethylated C's in CpG context: 146098875 Total unmethylated C's in CHG context: 153307574 Total unmethylated C's in CHH context: 657101999 Total unmethylated C's in Unknown context: 10034093 C methylated in CpG context: 2.7% C methylated in CHG context: 2.2% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 22.3% Bismark completed in 0d 6h 54m 58s