Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L001_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L001_R2_001.fastq.gz

Data processed at 11:17 on 2020-01-31


Alignment Stats

Sequence pairs analysed in total 113474961
Paired-end alignments with a unique best hit 18071283
Pairs without alignments under any condition 86568022
Pairs that did not map uniquely 8835656
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 979199135
Methylated C's in CpG context 4118192
Methylated C's in CHG context 3393802
Methylated C's in CHH context 15178693
Methylated C's in Unknown context 2887891
Unmethylated C's in CpG context 146098875
Unmethylated C's in CHG context 153307574
Unmethylated C's in CHH context 657101999
Unmethylated C's in Unknown context 10034093
Percentage methylation (CpG context) 2.7%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5072942 original top strand
CTOT 3933628 complementary to original top strand
CTOB 3960012 complementary to original bottom strand
OB 5104700 original bottom strand

Deduplication

Alignments analysed 18071282
Unique alignments 13855515
Duplicates removed 4215767
Duplicated alignments were found at 2908157 different positions

Cytosine Methylation after Extraction

Total C's analysed 491288104
Methylated C's in CpG context 1760890
Methylated C's in CHG context 1704605
Methylated C's in CHH context 7554564
Unmethylated C's in CpG context 72584544
Unmethylated C's in CHG context 76130851
Unmethylated C's in CHH context 331552650
Percentage methylation (CpG context) 2.4%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.2%

M-Bias Plot