Bismark report for: /gscratch/scrubbed/sr320/pg_all/Pg_val_1.fq.gz and /gscratch/scrubbed/sr320/pg_all/Pg_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v01/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 1237279577 Number of paired-end alignments with a unique best hit: 510447476 Mapping efficiency: 41.3% Sequence pairs with no alignments under any condition: 612597329 Sequence pairs did not map uniquely: 114234772 Sequence pairs which were discarded because genomic sequence could not be extracted: 86 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 252090696 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 258356694 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 15255999761 Total methylated C's in CpG context: 591642424 Total methylated C's in CHG context: 77274827 Total methylated C's in CHH context: 183983876 Total methylated C's in Unknown context: 3101698 Total unmethylated C's in CpG context: 1569847325 Total unmethylated C's in CHG context: 3194099749 Total unmethylated C's in CHH context: 9639151560 Total unmethylated C's in Unknown context: 32722537 C methylated in CpG context: 27.4% C methylated in CHG context: 2.4% C methylated in CHH context: 1.9% C methylated in unknown context (CN or CHN): 8.7% Bismark completed in 2d 21h 25m 4s