Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L002_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L002_R2_001.fastq.gz

Data processed at 21:11 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 81699034
Paired-end alignments with a unique best hit 4675527
Pairs without alignments under any condition 74543184
Pairs that did not map uniquely 2480323
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 261577090
Methylated C's in CpG context 1398442
Methylated C's in CHG context 1106576
Methylated C's in CHH context 5115414
Methylated C's in Unknown context 974335
Unmethylated C's in CpG context 40963331
Unmethylated C's in CHG context 42673164
Unmethylated C's in CHH context 170320163
Unmethylated C's in Unknown context 2569263
Percentage methylation (CpG context) 3.3%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2346837 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2328690 original bottom strand

Deduplication

Alignments analysed 4675527
Unique alignments 3944290
Duplicates removed 731237
Duplicated alignments were found at 528737 different positions

Cytosine Methylation after Extraction

Total C's analysed 134946990
Methylated C's in CpG context 608561
Methylated C's in CHG context 501154
Methylated C's in CHH context 2355542
Unmethylated C's in CpG context 21084295
Unmethylated C's in CHG context 22047792
Unmethylated C's in CHH context 88349646
Percentage methylation (CpG context) 2.8%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.6%

M-Bias Plot