Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L001_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F2_S22_L001_R2_001.fastq.gz

Data processed at 21:11 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 82177525
Paired-end alignments with a unique best hit 4815592
Pairs without alignments under any condition 74812200
Pairs that did not map uniquely 2549733
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 269443330
Methylated C's in CpG context 1442407
Methylated C's in CHG context 1137082
Methylated C's in CHH context 5281990
Methylated C's in Unknown context 1010601
Unmethylated C's in CpG context 42169766
Unmethylated C's in CHG context 43933031
Unmethylated C's in CHH context 175479054
Unmethylated C's in Unknown context 2663577
Percentage methylation (CpG context) 3.3%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2416982 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2398610 original bottom strand

Deduplication

Alignments analysed 4815592
Unique alignments 4022610
Duplicates removed 792982
Duplicated alignments were found at 570414 different positions

Cytosine Methylation after Extraction

Total C's analysed 137783479
Methylated C's in CpG context 623324
Methylated C's in CHG context 512711
Methylated C's in CHH context 2420387
Unmethylated C's in CpG context 21511795
Unmethylated C's in CHG context 22496198
Unmethylated C's in CHH context 90219064
Percentage methylation (CpG context) 2.8%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.6%

M-Bias Plot