Bismark report for: /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/caligus/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 112706276 Number of paired-end alignments with a unique best hit: 9883349 Mapping efficiency: 8.8% Sequence pairs with no alignments under any condition: 98036031 Sequence pairs did not map uniquely: 4786896 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4927716 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 4955633 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 534355619 Total methylated C's in CpG context: 2419146 Total methylated C's in CHG context: 1992060 Total methylated C's in CHH context: 9421424 Total methylated C's in Unknown context: 1713666 Total unmethylated C's in CpG context: 79153095 Total unmethylated C's in CHG context: 83267009 Total unmethylated C's in CHH context: 358102885 Total unmethylated C's in Unknown context: 5385442 C methylated in CpG context: 3.0% C methylated in CHG context: 2.3% C methylated in CHH context: 2.6% C methylated in unknown context (CN or CHN): 24.1% Bismark completed in 0d 6h 30m 34s