Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L002_R2_001.fastq.gz

Data processed at 21:11 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 112706276
Paired-end alignments with a unique best hit 9883349
Pairs without alignments under any condition 98036031
Pairs that did not map uniquely 4786896
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 534355619
Methylated C's in CpG context 2419146
Methylated C's in CHG context 1992060
Methylated C's in CHH context 9421424
Methylated C's in Unknown context 1713666
Unmethylated C's in CpG context 79153095
Unmethylated C's in CHG context 83267009
Unmethylated C's in CHH context 358102885
Unmethylated C's in Unknown context 5385442
Percentage methylation (CpG context) 3.0%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 2.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4927716 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4955633 original bottom strand

Deduplication

Alignments analysed 9883349
Unique alignments 7556502
Duplicates removed 2326847
Duplicated alignments were found at 1600864 different positions

Cytosine Methylation after Extraction

Total C's analysed 264271058
Methylated C's in CpG context 1000643
Methylated C's in CHG context 894871
Methylated C's in CHH context 4213533
Unmethylated C's in CpG context 38892968
Unmethylated C's in CHG context 41001021
Unmethylated C's in CHH context 178268022
Percentage methylation (CpG context) 2.5%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 2.3%

M-Bias Plot