Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L001_R1_001.fastq.gz and /gscratch/srlab/sr320/data/caligus/reads/raw/Sealice_F1_S20_L001_R2_001.fastq.gz

Data processed at 21:11 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 113474961
Paired-end alignments with a unique best hit 10187887
Pairs without alignments under any condition 98364893
Pairs that did not map uniquely 4922181
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 551028968
Methylated C's in CpG context 2494944
Methylated C's in CHG context 2053619
Methylated C's in CHH context 9741369
Methylated C's in Unknown context 1772643
Unmethylated C's in CpG context 81596268
Unmethylated C's in CHG context 85842553
Unmethylated C's in CHH context 369300215
Unmethylated C's in Unknown context 5582101
Percentage methylation (CpG context) 3.0%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 2.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5078535 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5109352 original bottom strand

Deduplication

Alignments analysed 10187887
Unique alignments 7700153
Duplicates removed 2487734
Duplicated alignments were found at 1695976 different positions

Cytosine Methylation after Extraction

Total C's analysed 269762284
Methylated C's in CpG context 1020695
Methylated C's in CHG context 914371
Methylated C's in CHH context 4308882
Unmethylated C's in CpG context 39679136
Unmethylated C's in CHG context 41823120
Unmethylated C's in CHH context 182016080
Percentage methylation (CpG context) 2.5%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 2.3%

M-Bias Plot