SUMMARISING RUN PARAMETERS ========================== Input filename: /Volumes/web/metacarcinus/Salmo_Calig/analyses/20190806_TrimGalore/Sealice_F1_S20_L002_R1_001_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection)) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All Read 1 sequences will be trimmed by 7 bp from their 5' end to avoid poor qualities or biases All Read 2 sequences will be trimmed by 7 bp from their 5' end to avoid poor qualities or biases (e.g. M-bias for BS-Seq applications) All Read 1 sequences will be trimmed by 7 bp from their 3' end to avoid poor qualities or biases All Read 2 sequences will be trimmed by 7 bp from their 3' end to avoid poor qualities or biases Output file will be GZIP compressed This is cutadapt 1.16 with Python 3.6.8 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /Volumes/web/metacarcinus/Salmo_Calig/analyses/20190806_TrimGalore/Sealice_F1_S20_L002_R1_001_val_1.fq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Traceback (most recent call last): File "/anaconda3/bin/cutadapt", line 11, in load_entry_point('cutadapt==1.16', 'console_scripts', 'cutadapt')() File "/anaconda3/lib/python3.6/site-packages/cutadapt/__main__.py", line 747, in main stats = runner.run() File "/anaconda3/lib/python3.6/site-packages/cutadapt/pipeline.py", line 174, in run (n, total1_bp, total2_bp) = self.process_reads() File "/anaconda3/lib/python3.6/site-packages/cutadapt/pipeline.py", line 216, in process_reads for read in self._reader: File "src/cutadapt/_seqio.pyx", line 201, in __iter__ (src/cutadapt/_seqio.c:5386) File "/anaconda3/lib/python3.6/gzip.py", line 289, in read1 return self._buffer.read1(size) File "/anaconda3/lib/python3.6/_compression.py", line 68, in readinto data = self.read(len(byte_view)) File "/anaconda3/lib/python3.6/gzip.py", line 469, in read buf = self._fp.read(io.DEFAULT_BUFFER_SIZE) File "/anaconda3/lib/python3.6/gzip.py", line 91, in read self.file.read(size-self._length+read) OSError: [Errno 5] Input/output error