Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_9_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_9_s1_R2_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 14 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 31847541 Number of paired-end alignments with a unique best hit: 16106014 Mapping efficiency: 50.6% Sequence pairs with no alignments under any condition: 9175850 Sequence pairs did not map uniquely: 6565677 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4344811 ((converted) top strand) GA/CT/CT: 3702414 (complementary to (converted) top strand) GA/CT/GA: 3705383 (complementary to (converted) bottom strand) CT/GA/GA: 4353401 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 446185263 Total methylated C's in CpG context: 48195285 Total methylated C's in CHG context: 2775999 Total methylated C's in CHH context: 12261277 Total methylated C's in Unknown context: 1257835 Total unmethylated C's in CpG context: 14395469 Total unmethylated C's in CHG context: 95974288 Total unmethylated C's in CHH context: 272582945 Total unmethylated C's in Unknown context: 5528605 C methylated in CpG context: 77.0% C methylated in CHG context: 2.8% C methylated in CHH context: 4.3% C methylated in unknown context (CN or CHN): 18.5% Bismark completed in 0d 7h 51m 54s