Alignment Stats
| Sequences analysed in total |
77350922 |
| Single-end alignments with a unique best hit |
25389646 |
| Sequences without alignments under any condition |
21586770 |
| Sequences that did not map uniquely |
30374506 |
| Genomic sequence context not extractable (edges of chromosomes) |
21762 |
|
|
Cytosine Methylation
| Total C's analysed |
150881047 |
| Methylated C's in CpG context |
16742773 |
| Methylated C's in CHG context |
575469 |
| Methylated C's in CHH context |
8126479 |
| Methylated C's in Unknown context |
3311 |
| Unmethylated C's in CpG context |
4068096 |
| Unmethylated C's in CHG context |
29027906 |
| Unmethylated C's in CHH context |
92340324 |
| Unmethylated C's in Unknown context |
25961 |
| Percentage methylation (CpG context) |
80.5% |
| Percentage methylation (CHG context) |
1.9% |
| Percentage methylation (CHH context) |
8.1% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
5701971 |
original top strand |
| CTOT |
7018609 |
complementary to original top strand |
| CTOB |
6974178 |
complementary to original bottom strand |
| OB |
5673126 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
25367884 |
| Unique alignments |
16680265 |
| Duplicates removed |
8687619 |
| Duplicated alignments were found at 3238822 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
97723805 |
| Methylated C's in CpG context |
9596337 |
| Methylated C's in CHG context |
406190 |
| Methylated C's in CHH context |
6301142 |
| Unmethylated C's in CpG context |
2711789 |
| Unmethylated C's in CHG context |
17840843 |
| Unmethylated C's in CHH context |
60867504 |
| Percentage methylation (CpG context) |
78.0% |
| Percentage methylation (CHG context) |
2.2% |
| Percentage methylation (CHH context) |
9.4% |
|
|
M-Bias Plot