Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs//zr1394_8_s456_trimmed.fq.gz

Data processed at 12:22 on 2019-03-31


Alignment Stats

Sequences analysed in total 38697189
Single-end alignments with a unique best hit 12100337
Sequences without alignments under any condition 11378609
Sequences that did not map uniquely 15218243
Genomic sequence context not extractable (edges of chromosomes) 10261
31.3%29.4%39.3%0.0265%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 74300289
Methylated C's in CpG context 10570191
Methylated C's in CHG context 337701
Methylated C's in CHH context 4071416
Methylated C's in Unknown context 2038
Unmethylated C's in CpG context 1613143
Unmethylated C's in CHG context 14787632
Unmethylated C's in CHH context 42920206
Unmethylated C's in Unknown context 13522
Percentage methylation (CpG context) 86.8%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 2680074 original top strand
CTOT 3383610 complementary to original top strand
CTOB 3361667 complementary to original bottom strand
OB 2664725 original bottom strand
OTCTOTCTOBOB00.5M1M1.5M2M2.5M3M3.5M
Number of Alignments

Deduplication

Alignments analysed 12090076
Unique alignments 8537033
Duplicates removed 3553043
Duplicated alignments were found at 1455924 different positions
70.6%29.4%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 51677627
Methylated C's in CpG context 6649597
Methylated C's in CHG context 247967
Methylated C's in CHH context 3261945
Unmethylated C's in CpG context 1131323
Unmethylated C's in CHG context 9857922
Unmethylated C's in CHH context 30528873
Percentage methylation (CpG context) 85.5%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 9.7%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

510152025300204060801000.2M0.4M0.6M0.8M1M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls