Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs//zr1394_13_s456_trimmed.fq.gz

Data processed at 12:22 on 2019-03-31


Alignment Stats

Sequences analysed in total 64352550
Single-end alignments with a unique best hit 19721209
Sequences without alignments under any condition 18442450
Sequences that did not map uniquely 26188891
Genomic sequence context not extractable (edges of chromosomes) 20168
30.6%28.6%40.7%0.0313%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 123608897
Methylated C's in CpG context 19125117
Methylated C's in CHG context 556295
Methylated C's in CHH context 6410972
Methylated C's in Unknown context 3425
Unmethylated C's in CpG context 3215880
Unmethylated C's in CHG context 24861808
Unmethylated C's in CHH context 69438825
Unmethylated C's in Unknown context 22722
Percentage methylation (CpG context) 85.6%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 8.5%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 4410505 original top strand
CTOT 5484879 complementary to original top strand
CTOB 5435057 complementary to original bottom strand
OB 4370600 original bottom strand
OTCTOTCTOBOB01M2M3M4M5M
Number of Alignments

Deduplication

Alignments analysed 19701041
Unique alignments 11234204
Duplicates removed 8466837
Duplicated alignments were found at 2615165 different positions
57%43%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 68890041
Methylated C's in CpG context 9111111
Methylated C's in CHG context 344677
Methylated C's in CHH context 4621384
Unmethylated C's in CpG context 1824835
Unmethylated C's in CHG context 12907020
Unmethylated C's in CHH context 40081014
Percentage methylation (CpG context) 83.3%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 10.3%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

510152025300204060801000.2M0.4M0.6M0.8M1M1.2M1.4M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls