Alignment Stats
| Sequences analysed in total |
64352550 |
| Single-end alignments with a unique best hit |
19721209 |
| Sequences without alignments under any condition |
18442450 |
| Sequences that did not map uniquely |
26188891 |
| Genomic sequence context not extractable (edges of chromosomes) |
20168 |
|
|
Cytosine Methylation
| Total C's analysed |
123608897 |
| Methylated C's in CpG context |
19125117 |
| Methylated C's in CHG context |
556295 |
| Methylated C's in CHH context |
6410972 |
| Methylated C's in Unknown context |
3425 |
| Unmethylated C's in CpG context |
3215880 |
| Unmethylated C's in CHG context |
24861808 |
| Unmethylated C's in CHH context |
69438825 |
| Unmethylated C's in Unknown context |
22722 |
| Percentage methylation (CpG context) |
85.6% |
| Percentage methylation (CHG context) |
2.2% |
| Percentage methylation (CHH context) |
8.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
4410505 |
original top strand |
| CTOT |
5484879 |
complementary to original top strand |
| CTOB |
5435057 |
complementary to original bottom strand |
| OB |
4370600 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
19701041 |
| Unique alignments |
11234204 |
| Duplicates removed |
8466837 |
| Duplicated alignments were found at 2615165 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
68890041 |
| Methylated C's in CpG context |
9111111 |
| Methylated C's in CHG context |
344677 |
| Methylated C's in CHH context |
4621384 |
| Unmethylated C's in CpG context |
1824835 |
| Unmethylated C's in CHG context |
12907020 |
| Unmethylated C's in CHH context |
40081014 |
| Percentage methylation (CpG context) |
83.3% |
| Percentage methylation (CHG context) |
2.6% |
| Percentage methylation (CHH context) |
10.3% |
|
|
M-Bias Plot