Alignment Stats
| Sequences analysed in total |
59459431 |
| Single-end alignments with a unique best hit |
20145696 |
| Sequences without alignments under any condition |
17101979 |
| Sequences that did not map uniquely |
22211756 |
| Genomic sequence context not extractable (edges of chromosomes) |
16457 |
|
|
Cytosine Methylation
| Total C's analysed |
120343086 |
| Methylated C's in CpG context |
13832027 |
| Methylated C's in CHG context |
461081 |
| Methylated C's in CHH context |
6664085 |
| Methylated C's in Unknown context |
2655 |
| Unmethylated C's in CpG context |
3413355 |
| Unmethylated C's in CHG context |
24010493 |
| Unmethylated C's in CHH context |
71962045 |
| Unmethylated C's in Unknown context |
19915 |
| Percentage methylation (CpG context) |
80.2% |
| Percentage methylation (CHG context) |
1.9% |
| Percentage methylation (CHH context) |
8.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
4509629 |
original top strand |
| CTOT |
5594115 |
complementary to original top strand |
| CTOB |
5549860 |
complementary to original bottom strand |
| OB |
4475635 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
20129239 |
| Unique alignments |
13660276 |
| Duplicates removed |
6468963 |
| Duplicated alignments were found at 2609514 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
80756458 |
| Methylated C's in CpG context |
8385192 |
| Methylated C's in CHG context |
335216 |
| Methylated C's in CHH context |
5285129 |
| Unmethylated C's in CpG context |
2342276 |
| Unmethylated C's in CHG context |
15323134 |
| Unmethylated C's in CHH context |
49085511 |
| Percentage methylation (CpG context) |
78.2% |
| Percentage methylation (CHG context) |
2.1% |
| Percentage methylation (CHH context) |
9.7% |
|
|
M-Bias Plot