Bismark report for: /gscratch/srlab/sr320/data/olurida-bs//zr1394_10_s456_trimmed.fq.gz (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 14 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 78221457 Number of alignments with a unique best hit from the different alignments: 25462569 Mapping efficiency: 32.6% Sequences with no alignments under any condition: 21496817 Sequences did not map uniquely: 31262071 Sequences which were discarded because genomic sequence could not be extracted: 30186 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 6528162 ((converted) top strand) CT/GA: 6453340 ((converted) bottom strand) GA/CT: 6260193 (complementary to (converted) top strand) GA/GA: 6190688 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 159765962 Total methylated C's in CpG context: 24639901 Total methylated C's in CHG context: 738394 Total methylated C's in CHH context: 8517097 Total methylated C's in Unknown context: 4229 Total unmethylated C's in CpG context: 4431826 Total unmethylated C's in CHG context: 33913328 Total unmethylated C's in CHH context: 87525416 Total unmethylated C's in Unknown context: 27106 C methylated in CpG context: 84.8% C methylated in CHG context: 2.1% C methylated in CHH context: 8.9% C methylated in Unknown context (CN or CHN): 13.5% Bismark completed in 0d 5h 37m 12s