FastQCFastQC Report
Mon 24 Feb 2025
POC-259-TP3_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-259-TP3_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21755938
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA454530.20892227216312162No Hit
ACGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATG266810.12263778284347013No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA239580.11012165965907791No Hit
GTGACTCGGCAGTGTTCGGCTTATAAAACGTCAAGGTCTTGTAAAACAGC226000.10387968562881546No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT218770.10055645497794671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)