FastQCFastQC Report
Mon 24 Feb 2025
POC-219-TP4_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP4_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19082344
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA391090.20494861637543063No Hit
ACGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATG268580.14074790811862525No Hit
AATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAAC235420.1233705880158119No Hit
AAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTT234750.12301947811023635No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT226510.11870135031629239No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA222520.11661041222189475No Hit
ACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTT218020.11425221136355157No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA208800.10942051982712397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)