FastQCFastQC Report
Mon 24 Feb 2025
POC-219-TP3_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP3_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20948211
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTT4972162.3735487483871536No Hit
AACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATT3509711.6754223069454475No Hit
CAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTT1706200.8144848264131003No Hit
TATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTTT1642940.7842865436098576No Hit
ACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAG1228790.5865846968984607No Hit
AAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAG1203290.5744118197014533No Hit
TTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAA1174400.5606206658888436No Hit
CTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGT1137150.5428387178265486No Hit
TTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCAC1098650.5244600600977334No Hit
AGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAA1040770.49683001569919266No Hit
TAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTA1024590.48910620577575814No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT956470.45658791578908575No Hit
TAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGA794210.3791302273974613No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT784210.3743565500653015No Hit
CGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAA762040.3637733074199033No Hit
TTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTT692370.3305150974467462No Hit
TACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTA573470.27375607396736645No Hit
TCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAATGTTTTTCTTTTTTTT571110.27262948611697674No Hit
TTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAAAAACTT519510.24799731108303236No Hit
GGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCA519130.24781591134441028No Hit
AATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTT514930.24581096686490314No Hit
GAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAA490850.23431595184906243No Hit
GTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAG476930.22767099300269605No Hit
TTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAAC473690.2261243215470763No Hit
TTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTTTT451590.21557449464300318No Hit
TACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCA447530.21363638164614632No Hit
TTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGC427590.2041176690458197No Hit
CAGTGTTACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACA419290.2001555168601271No Hit
TATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACA417410.19925806552168107No Hit
TTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTAT410940.19616949628777372No Hit
ATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTC406580.19408817297095202No Hit
ATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACAC403720.19272290125395433No Hit
TCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAA392560.18739547735126405No Hit
AATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTA389070.18572946396234027No Hit
TTGACAATTGATTACGCTACATTATCACAGTCAGTGTTACCGCGGCCATT374870.1789508421506734No Hit
GCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAA372570.17785289636427665No Hit
CAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCA372370.17775742281763346No Hit
TAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAAAAA359130.1714370740298539No Hit
TGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAACTTTTAT351300.1676992846787728No Hit
TTATTAAAAAATAAATCCTTAAGTCTTACCGCGTCGGCTGGCACTTAATT344240.164329068482268No Hit
GTTGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAACTTTT335760.1602809901045965No Hit
TTTGCTCACACAATTCATGATTAAATCACTTGGTTTCGGGAAATTTGACT332720.15882979219561996No Hit
AACAAACATATTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAAA328270.15670550578280887No Hit
GTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAATGTC327290.1562376854042572No Hit
CTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATA319250.15239964882920073No Hit
ATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTTTG314770.15026104138439314No Hit
TAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGCT307960.14701016712119236No Hit
CTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAAAAACT306370.14625115242537895No Hit
ATTTCAAAGTTAAACATGCGTCATAGCTCATTTTCGTTACTTTTTAAAAA303190.14473312303375216No Hit
ACATTTTTATTGTCTAAATTTAATTTTTTTTTAAACTAAAAAAAAAAAAA302270.14429394471919343No Hit
TTTTGCTCACACAATTCATGATTAAATCACTTGGTTTCGGGAAATTTGAC299910.14316735686880375No Hit
ACAATTGATTACGCTACATTATCACAGTCAGTGTTACCGCGGCCATTTAA298640.14256109984761944No Hit
TTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAA298270.1423844737863295No Hit
CTCTTTTAACAAAAAATCTTTTCATTTATTGTTTGTTTACTTTTTTTTTA297660.14209327946906777No Hit
TTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCTTCAGAAAAGT291570.13918610997378247No Hit
ACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAG288250.13760124909950544No Hit
GATTCAACCATCGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATG286550.1367897239530383No Hit
GTCAGTGTTACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAA284660.1358874989372601No Hit
TCAACCATCGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTT284110.1356249466839913No Hit
AAACCAACCCTTACGAGTGACTACACCGTAGGACGTTACAATTCAACATC280450.13387778078042084No Hit
CGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAGTG276870.13216880429550762No Hit
AATAACGGATCAAAAAACACACCAAAAATGTCTCATCAATAAAAAAATTT274980.1312665792797294No Hit
TCTTTTAACAAAAAATCTTTTCATTTATTGTTTGTTTACTTTTTTTTTAT273110.13037390161861553No Hit
TAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCACC263250.125667055769106No Hit
ATTAAAAAATAAATCCTTAAGTCTTACCGCGTCGGCTGGCACTTAATTTG262430.1252756142278689No Hit
TTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAA260940.12456433630537711No Hit
TTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAA258790.12353799567896276No Hit
TACGAGTGACTACACCGTAGGACGTTACAATTCAACATCGAGGTCGCAAA258300.12330408548968691No Hit
TAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAA257700.12301766484975733No Hit
AGATTCAACCATCGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTAT257650.12299379646309654No Hit
ACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTAT254450.12146621971680541No Hit
CCTTTACAGTACTTTCATTATCAGTATAAACTAATGTTTAAGCAAGATCT249380.11904596530940041No Hit
AATTTCACTCTCCACTACTCTCGGAATCTCGTTTGATTTTTACTTAGTAA248250.11850653977086634No Hit
AATTTCATTGAATATTTTTTTAAGACAGAAAAACTTTCGGGATACCATTC246450.11764727785107758No Hit
CTGAGATTTTTGCCACAATCACCAGTTTTTGCTCACACAATTCATGATTA244870.11689303683259636No Hit
CACCCCTAATCACAAATTTTCTGAGATTTTTGCCACAATCACCAGTTTTT241350.11521270241167611No Hit
ATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGAC239650.11440117726520896No Hit
TAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCG236630.11295952671089671No Hit
GATTAGCCTTTCACCCCTAATCACAAATTTTCTGAGATTTTTGCCACAAT236310.11280676903626759No Hit
ACTCTCCACTACTCTCGGAATCTCGTTTGATTTTTACTTAGTAATAAGAT231180.1103578725648696No Hit
AAAAAATTACTTAAAATTTTCCATTTTAAATATGCCAAATAAATCTCTTT230180.10988050483165364No Hit
TCTCTTTTAACAAAAAATCTTTTCATTTATTGTTTGTTTACTTTTTTTTT229410.10951293167707735No Hit
CAGATTTAATTTCATTGAATATTTTTTTAAGACAGAAAAACTTTCGGGAT224700.10726452965363008No Hit
GTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAA224010.10693514591771107No Hit
CAACCATCGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTT222070.10600905251527207No Hit
TGTCACCACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAA219830.10493974879286828No Hit
ACTCAAATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGT218870.10448147576898094No Hit
ACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAA217340.1037511031371605No Hit
TGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAAC216760.10347422985189524No Hit
ACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTT216330.10326896172661237No Hit
TTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACC213940.10212805284422619No Hit
TGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCA211410.10092031247918976No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)