FastQCFastQC Report
Mon 24 Feb 2025
POC-219-TP2_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP2_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18547378
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATG230340.12419006071909464No Hit
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA229420.1236940337335013No Hit
ACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTT188280.10151300092120838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)