FastQCFastQC Report
Mon 24 Feb 2025
POC-201-TP3_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-201-TP3_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15829437
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA1024360.6471234573914411No Hit
ACGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATG744610.4703957569684885No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA651860.4118023906977866No Hit
AAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTT638200.4031728986950073No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT624810.3947139749821803No Hit
AATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAAC620720.3921301812566044No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA565720.3573847888588836No Hit
TCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTT502450.3174149529133601No Hit
ACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTT481080.3039147886308275No Hit
TGCTTGTGGTGGTCATAGATCTTGCTTAAACATTAGTTTATACTGATAAT479190.30272081060116035No Hit
ACGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACATTTTTGGTGTG469470.29658035216287226No Hit
GAAATTTTTTTATTGATGAGACATTTTTGGTGTGTTTTTTGATCCGTTAT438890.2772619139897395No Hit
CATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAG415120.2622455871298518No Hit
CAGTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTG412330.2604830481336765No Hit
TAATAAAGACAATTAAATGGCCGCGGTAACACTGACTGTGATAATGTAGC371280.23455035071683217No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA370510.23406391522326409No Hit
GACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCTTCAG314890.19892684749305994No Hit
AAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTT308060.19461210149167024No Hit
ATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCT305370.19291273593621808No Hit
TAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCGG297650.18803574631239253No Hit
ATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATAC296800.18749877206624593No Hit
CTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAG294040.18575518510228758No Hit
TAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGA288810.18245121415246798No Hit
TTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCTTCAGAAAAGT280610.1772709920131714No Hit
CTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCG280170.17699302887398966No Hit
ATTTTTTATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAA271480.17150325687514975No Hit
CAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAGCTCAAATCAGAAGCT260040.16427621525642386No Hit
GAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTA249680.15773144679750772No Hit
TAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAATATCGG248850.15720710723950573No Hit
GAGCTTTACTGTAAGCTTATATATATAATTTTTTTTTTTTTATAAGTAAG247070.15608261999463405No Hit
GATTACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAAATTTTTTATG241070.15229221355124634No Hit
TTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAA236380.1493293791813316No Hit
GAGTTGTGCTTGTGGTGGTCATAGATCTTGCTTAAACATTAGTTTATACT235060.1484954897637863No Hit
ATTAAATCTGTAGTGAAGATGCTACATTTTAATTGTTAGACGAGAAGTCC230300.14548843398536537No Hit
TTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAG229840.14519783615803897No Hit
ATATATATAATTTTTTTTTTTTTATAAGTAAGACAGTTTTGTTGGGGCGA226130.14285410150721092No Hit
AATTAATTGTTGACCGGTATGAATGGTATCCCGAAAGTTTTTCTGTCTTA222740.14071252186669683No Hit
ATCAATTGTCAATTAATTGTTGACCGGTATGAATGGTATCCCGAAAGTTT222730.14070620452262453No Hit
TTTCTGTCTTAAAAAAATATTCAATGAAATTAAATCTGTAGTGAAGATGC220480.13928480210635413No Hit
AACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAA220290.1391647725689802No Hit
TTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACA214390.1354375395663156No Hit
CTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGT207400.1310217160597689No Hit
TTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACAT203120.128317892796819No Hit
GAAATAGCTGGTTTTCTGCGAAAACTATTTAAGTAGTGTTCTTTTTTTTT202980.1282294499798066No Hit
TGATCCGTTATTTTGAATGAAAAAAATAACGAAAACAAATAAAAGTTACC199890.12627739066146193No Hit
AAAAATATTCAATGAAATTAAATCTGTAGTGAAGATGCTACATTTTAATT197580.12481808418075765No Hit
AGTTGAAAGGAATTTGAATCTTTTATTTTTGAAGTAGCTTTAAAAAAGCG195200.1233145562915472No Hit
AATCTTTTATTTTTGAAGTAGCTTTAAAAAAGCGTACCTTTTGTATAATG193940.12251857093843578No Hit
ACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACC191150.12075603194226048No Hit
TAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGT190120.12010534550281227No Hit
TTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCG186350.1177237067875503No Hit
AAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTT185410.1171298764447529No Hit
GTTACCCTGGGGATAACAGCGCAATAATGTTTGAGAGTTAATCAACGACA177020.11182962476808239No Hit
TGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATG173350.10951115949354358No Hit
AACTTTGAAATTTTTTTATTGATGAGACATTTTTGGTGTGTTTTTTGATC169280.10694000045611224No Hit
ATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATGGT164590.1039771660861975No Hit
TTAAAAAAATATTCAATGAAATTAAATCTGTAGTGAAGATGCTACATTTT162270.10251154226142092No Hit
TTAATTGTTAGACGAGAAGTCCCCATGGAGCTTTACTGTAAGCTTATATA161140.10179768238124957No Hit
AACATTAGTTTATACTGATAATGAAAGTACTGTAAAGGAAAGTTGAAAGA160060.10111540922143977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)