FastQCFastQC Report
Thu 20 Feb 2025
POC-53-TP4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-53-TP4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20021973
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1719270.858691598475335No Hit
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA361590.18059658755907823No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA221400.11057851291678399No Hit
CGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTA207060.10341638159236356No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT204910.10234256134497835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)