FastQCFastQC Report
Thu 20 Feb 2025
POC-52-TP1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-52-TP1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16402600
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1775561.0824869228049212No Hit
GAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTAC733440.44714862277931544No Hit
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA561320.34221403923768184No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA434530.26491531830319587No Hit
ATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAAC426780.26019045761037884No Hit
AAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTT398840.2431565727384683No Hit
AAAAAAGTAAACAAACAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAG397420.24229085632765535No Hit
AAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATT317640.19365222586663092No Hit
TTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAG272220.16596149390950216No Hit
AAAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAA264860.1614744003999366No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248730.1516405935644349No Hit
GGAAAAGAAAAAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAA248320.15139063319229878No Hit
CGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTA234580.14301391242851744No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT230420.1404777291405021No Hit
TTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAG220040.1341494641093485No Hit
GGCTAGATGAGCAGAGAAGACCCCCGAAGAGCATCACCCCAACCCCAAAG199950.12190140587467839No Hit
AGGACGAGGATGGAGATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAG198820.12121249070269347No Hit
GTTCAGTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTT189870.11575603867679514No Hit
AAAAAAAGTAAACAAACAATAAATGAAAAGATTTTTTGTTAAAAGAGAAA187010.11401241266628462No Hit
ATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTT184970.11276870740004634No Hit
GTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGG169760.10349578725324032No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)