FastQCFastQC Report
Thu 20 Feb 2025
POC-42-TP4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-42-TP4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16179587
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA522800.32312320456634647No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA522130.32270910252529933No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA481330.29749214241377114No Hit
GAAAAACTTTCGGGATACCATTCATACCGGTCAACAATTAATTGACAATT320760.1982498069944554No Hit
CTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTA232960.1439838977348433No Hit
CGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTC192610.1190450658598393No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT188160.11629468663198882No Hit
CTACATTATCACAGTCAGTGTTACCGCGGCCATTTAATTGTCTTTATTAG179270.11080010880376613No Hit
GCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAG173230.10706700980686343No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)