FastQCFastQC Report
Thu 20 Feb 2025
POC-259-TP3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-259-TP3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22149153
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1098980.49617247214825777No Hit
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA468260.21141214745322318No Hit
GTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTGAA269790.12180601217572518No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA243740.11004484008937047No Hit
CGGCGGAGTTCCTGCGTGACTCGGCAGTGTTCGGCTTATAAAACGTCAAG225690.10189554426753926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)