FastQCFastQC Report
Thu 20 Feb 2025
POC-255-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-255-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24339256
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA723640.2973139359724061No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA589730.2422958203816912No Hit
CTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTA498270.2047186651884511No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA487820.20042518966068643No Hit
GTCGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATC369880.15196849073776125No Hit
CGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTG352770.14493869492148817No Hit
CACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAAC350250.1439033304879985No Hit
CCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAAC329960.13556700336279795No Hit
CGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATCCC314500.12921512473511929No Hit
CCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACG313800.12892752350359435No Hit
GTCATAGCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAAC311480.12797433085054039No Hit
GCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAG310070.12739501979846876No Hit
GAAAAACTTTCGGGATACCATTCATACCGGTCAACAATTAATTGACAATT296360.12176214424960237No Hit
TTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAAC292490.1201721202981718No Hit
CTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCGTAGGACG292410.12013925158599753No Hit
CGCTGTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATT287310.1180438711848875No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT279660.11490080058322243No Hit
CTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAA277450.11399280240940808No Hit
CTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATT257660.10586190473529675No Hit
ATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAATGTCTCA255550.10499499245170026No Hit
GTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAA254220.10444855011180292No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACGTCATCTAGGGG252410.10370489549885996TruSeq Adapter, Index 1 (97% over 39bp)
AAACAATCCTAATTTAAAGAATAGTTATATTAAGAGAACTATTGATTTTT250480.10291193781765556No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)