FastQCFastQC Report
Thu 20 Feb 2025
POC-219-TP3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21473974
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA3087481.4377776558731048No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA2346161.092559765602771No Hit
TTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAG1467060.683180486294712No Hit
CGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTA1368880.6374600248654488No Hit
GAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTAC1341000.6244768667411071No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT1282560.597262528118922No Hit
TTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAG1214840.5657266791884911No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1119360.5212635537325322No Hit
GTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGG1086090.5057703804614833No Hit
GGATAACTAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTC1073330.4998283037876455No Hit
GTTCAGTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTT1069560.4980726902249206No Hit
ATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAAC976220.45460611994780287No Hit
ATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTT940820.4381210482978139No Hit
TTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGT878000.4088670313189352No Hit
GATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTAT806200.37543120802884455No Hit
CTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTG787880.3668999506099802No Hit
GTGAAATTGGAGTTGTGCTTGTGGTGGTCATAGATCTTGCTTAAACATTA758060.3530133733048201No Hit
TTCAGTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTC753390.3508386477509938No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA746200.34749040862208364No Hit
AAAAAAGTAAACAAACAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAG717170.33397171850911245No Hit
AAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTT707410.3294266818056127No Hit
ATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAAA687230.3200292596051387No Hit
CTAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGC681840.3175192444584314No Hit
AAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATT610730.2844047403615186No Hit
GTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTGAA531280.24740646514706593No Hit
GGGTAATACAGTTTCTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAA515180.23990901730625175No Hit
CGACTGTTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACC511750.23831173494016522No Hit
GTTTCTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAAT490340.22834152635185273No Hit
CTTCGACTGTTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAA486220.22642292479258846No Hit
GTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGG460950.214655191442441No Hit
AAAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAA442850.20622638362140142No Hit
GGAAAAGAAAAAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAA439990.20489453884967915No Hit
TGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATG423290.19711768301479737No Hit
AAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACT402630.18749673441906933No Hit
AAAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGAC389340.18130784735047178No Hit
ATTAAATGGCCGCGGTAACACTGACTGTGATAATGTAGCGTAATCAATTG378890.17644149145379426No Hit
GTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGAT378310.17617139705952892No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376170.17517484188068777No Hit
TGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGA362360.16874380121723162No Hit
TTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAA360810.16802199723255695No Hit
AAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGT342940.15970029580924333No Hit
CGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATGA339500.1580983566432557No Hit
AAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAATATCGGG337100.15698072466698526No Hit
AAAAAAAGTAAACAAACAATAAATGAAAAGATTTTTTGTTAAAAGAGAAA332570.15487119431177482No Hit
AAAATATTTAGTCTAGGTTATACATGCTAGTGTATGCGAACAGGTGAGGA327200.15237049276486972No Hit
CGCGGTAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATT324250.1509967367940373No Hit
AATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCT311740.14517108011772764No Hit
CGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACATTTTTGGTGTGT309040.1439137441444234No Hit
TGGAGAGTGAAATTGGAGTTGTGCTTGTGGTGGTCATAGATCTTGCTTAA305320.1421814145812042No Hit
AAAAAAGAGGATAACTAAGATAAACTAAAATTAGACGATTTTTTTCGAAA303580.14137113139840812No Hit
AAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTT292180.13606237951112354No Hit
CGGTAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGT290420.13524278272852525No Hit
ATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTAC288980.13457220354276297No Hit
GGAAAGTTGAAAGAGAGTTGAAAGGAATTTGAATCTTTTATTTTTGAAGT287920.13407858275324352No Hit
CGAGAAGTCCCCATGGAGCTTTACTGTAAGCTTATATATATAATTTTTTT286060.13321241797163394No Hit
AGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTA284210.13235090998992546No Hit
GTTTTGTTGGGGCGACAGTTTTTTAAAAAGTAACGAAAATGAGCTATGAC283330.13194111159862631No Hit
GCCCGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAA278060.1294869780507325No Hit
AAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAAC276580.1287977716653657No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA274970.1280480268812843No Hit
GCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATGGTATCCCGAA264180.12302333978796845No Hit
GTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTTTTATAAAA261360.12171012221585067No Hit
TTTCTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAATA257850.12007558545055518No Hit
TAAATGGCCGCGGTAACACTGACTGTGATAATGTAGCGTAATCAATTGTC257470.11989862705431234No Hit
GTTTTTCTGTCTTAAAAAAATATTCAATGAAATTAAATCTGTAGTGAAGA255210.11884619027665769No Hit
AAAAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGA253540.11806850469316951No Hit
CCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAAATTTTTTATGAAGAT253480.11804056389376275No Hit
GTTGAAAGGAATTTGAATCTTTTATTTTTGAAGTAGCTTTAAAAAAGCGT248030.11550260794764863No Hit
CAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAGCTCAAATCAGAAGCT247670.11533496315120806No Hit
CATAGATCTTGCTTAAACATTAGTTTATACTGATAATGAAAGTACTGTAA244750.11397517758007902No Hit
GTAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTG241240.11234064081478351No Hit
GTGAAACCTGCCCGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAA235980.1098911640667908No Hit
AATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTA234830.10935563207816124No Hit
CTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAATATCG233400.10868970969230007No Hit
CCGCGGTAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAAT231880.10798187610732882No Hit
AAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTT230870.107511539317315No Hit
GGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTTTT230690.10742771691909471No Hit
GTTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCC227960.10615641054608708No Hit
TTTTTATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAAAA227570.10597479534994315No Hit
ATTTTTTATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAA225820.10515985536724597No Hit
AAAATATTCAATGAAATTAAATCTGTAGTGAAGATGCTACATTTTAATTG225640.10507603296902567No Hit
TCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGG225150.10484784977387045No Hit
CTTGCTTAAACATTAGTTTATACTGATAATGAAAGTACTGTAAAGGAAAG220470.1026684674201431No Hit
TTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGA219500.1022167578297338No Hit
AAAAAAAGAGGATAACTAAGATAAACTAAAATTAGACGATTTTTTTCGAA217700.10137853384753097No Hit
GTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAAT216940.10102461705504534No Hit
GAAAGTTTTTCTGTCTTAAAAAAATATTCAATGAAATTAAATCTGTAGTG216920.10101530345524308No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)