FastQCFastQC Report
Thu 20 Feb 2025
POC-219-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18890758
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA251450.13310741686490293No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA214010.11328820156396052No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTTCGAATCTGGGGG208590.11041907370789461TruSeq Adapter, Index 14 (97% over 40bp)
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA198570.10511489268985395No Hit
CTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTA197450.1045220101808514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)