FastQCFastQC Report
Thu 20 Feb 2025
POC-201-TP3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-201-TP3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16212213
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA1053210.6496398733473339No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG940010.5798159696026692No Hit
GTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTGAA754640.4654762431260927No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA665230.41032646190868577No Hit
GAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTAC639480.3944433742635876No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT631540.38954583189845826No Hit
CGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTA621360.38326661511293986No Hit
GTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGG562430.3469174751158278No Hit
ATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAAC502380.30987749790852115No Hit
GGATAACTAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTC482490.297608969238191No Hit
GTGAAATTGGAGTTGTGCTTGTGGTGGTCATAGATCTTGCTTAAACATTA476330.2938093645821209No Hit
CGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATGA467780.2885355626650106No Hit
CGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACATTTTTGGTGTGT443110.2733186394726001No Hit
TTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAG423740.2613708566498602No Hit
GTTTTGTTGGGGCGACAGTTTTTTAAAAAGTAACGAAAATGAGCTATGAC412400.25437612989664027No Hit
GTTCAGTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTT403720.2490221415176324No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA373420.23033252770611884No Hit
CGAGAAGTCCCCATGGAGCTTTACTGTAAGCTTATATATATAATTTTTTT354760.21882268632912732No Hit
AAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTT316290.19509366179681947No Hit
GATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTAT314010.1936873146189234No Hit
CTTCGACTGTTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAA309270.19076359285435No Hit
CTAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGC304970.188111271422353No Hit
ATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTT301800.18615595538992732No Hit
TTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAG298730.184262321251269No Hit
AAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATT296360.18280046036898231No Hit
ATTAAATGGCCGCGGTAACACTGACTGTGATAATGTAGCGTAATCAATTG292970.1807094441702684No Hit
CTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTG280650.17311023485812826No Hit
GTTTCTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAAT277990.17146949648391616No Hit
AAAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAA262160.16170525270054126No Hit
AAAAAAGTAAACAAACAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAG258800.15963274106995756No Hit
GTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGG256880.15844844870962405No Hit
ATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAAA252210.15556790427068778No Hit
GGGTAATACAGTTTCTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAA249640.15398267960086634No Hit
GGAAAAGAAAAAAAAAAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAA248070.1530142738687186No Hit
TTCAGTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTC233710.14415675392372404No Hit
AAAATATTCAATGAAATTAAATCTGTAGTGAAGATGCTACATTTTAATTG231460.1427689113139582No Hit
CTTTACTGTAAGCTTATATATATAATTTTTTTTTTTTTATAAGTAAGACA227530.14034481288890047No Hit
TGGAGAGTGAAATTGGAGTTGTGCTTGTGGTGGTCATAGATCTTGCTTAA225420.13904332493040894No Hit
TGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATG223250.137704827835657No Hit
GCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATGGTATCCCGAA222090.13698931786795548No Hit
GGTATCCCGAAAGTTTTTCTGTCTTAAAAAAATATTCAATGAAATTAAAT220380.13593455748453342No Hit
CGACTGTTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACC220280.13587287559076605No Hit
GCCCGATGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAA207340.1278912385372682No Hit
AAAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGAC207240.1278295566435008No Hit
TTTTGGTGTGTTTTTTGATCCGTTATTTTGAATGAAAAAAATAACGAAAA200410.12361668329918933No Hit
GGCTAATCGAACTTGGAAATAGCTGGTTTTCTGCGAAAACTATTTAAGTA199810.12324659193658509No Hit
AAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACT199430.12301220074026908No Hit
GTTTTTCTGTCTTAAAAAAATATTCAATGAAATTAAATCTGTAGTGAAGA198220.12226584982568389No Hit
GGAAAGTTGAAAGAGAGTTGAAAGGAATTTGAATCTTTTATTTTTGAAGT195310.12047090671705336No Hit
GTTGAAAGGAATTTGAATCTTTTATTTTTGAAGTAGCTTTAAAAAAGCGT195220.12041539301266273No Hit
TAAATGGCCGCGGTAACACTGACTGTGATAATGTAGCGTAATCAATTGTC193540.11937913719737091No Hit
TTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGT192420.11868829998717632No Hit
GTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTTTTATAAAA186690.11515392747430594No Hit
CGAAAACAAATAAAAGTTACCCTGGGGATAACAGCGCAATAATGTTTGAG176990.1091707837788709No Hit
CGGTAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGT174630.10771509108596093No Hit
CTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACATTTTTG171040.10550071109971229No Hit
GTAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTG165250.10192932945058147No Hit
GAAAGTTTTTCTGTCTTAAAAAAATATTCAATGAAATTAAATCTGTAGTG162820.10043045943203435No Hit
TGAAGATGCTACATTTTAATTGTTAGACGAGAAGTCCCCATGGAGCTTTA162320.1001220499631975No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)