FastQCFastQC Report
Thu 20 Feb 2025
POC-201-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-201-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16212213
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA1041090.6421640278227284No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA844030.520613687964746No Hit
CTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTA673990.4157297958027075No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA670140.41335504289266367No Hit
GTCGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATC555570.3426860972033861No Hit
CGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTG544990.3361601528427982No Hit
CACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAAC537370.3314599925377245No Hit
CGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATCCC504630.311265340518287No Hit
CTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCGTAGGACG476450.2938833828546417No Hit
CCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAAC475850.29351329149203753No Hit
CCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACG465140.286907160669552No Hit
TTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAAC461990.28496418101587984No Hit
CGCTGTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATT459150.2832124152328864No Hit
GCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAG433840.2676007279203647No Hit
CTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAA420420.25932301777678346No Hit
GTCATAGCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAAC413400.25499294883431395No Hit
GAACAAACCAACCCTTACGAGTGACTACACCGTAGGACGTTACAATTCAA397020.24488945463521852No Hit
ATAGAAACCAAGCTGTGTCACCACGCTTTTAACTCAAATCATGTATAACT380210.23452072829292336No Hit
GTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAA375570.23165868842211731No Hit
GAAAAACTTTCGGGATACCATTCATACCGGTCAACAATTAATTGACAATT369330.22780973825103334No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT360690.22248042262953244No Hit
ATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAATGTCTCA359260.22159837154865905No Hit
GTCACCACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAA356030.2196060463799729No Hit
AAACAATCCTAATTTAAAGAATAGTTATATTAAGAGAACTATTGATTTTT347310.21422738524345813No Hit
GGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAAT332940.20536369710908686No Hit
CTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATT332040.2048085600651805No Hit
GCTGGTTCCAAGCCTACATTTTTATTGTCTAAATTTAATTTTTTTTTAAA326940.20166278348304453No Hit
CTCAAATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTG311510.1921452672747391No Hit
CTCATCAATAAAAAAATTTCAAAGTTAAACATGCGTCATAGCTCATTTTC308070.19002341012914153No Hit
CGAGGTCGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGT301100.18572418213355574No Hit
CCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGG283140.1746461140129358No Hit
GGATGAACAAACCAACCCTTACGAGTGACTACACCGTAGGACGTTACAAT274110.16907623900574215No Hit
GTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAA266970.16467215179075181No Hit
ATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTT264870.16337683202163703No Hit
CGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTC261170.16109460195224426No Hit
CTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATA257300.158707512663447No Hit
CTGTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCA248730.15342137436758324No Hit
CAAAAATGTCTCATCAATAAAAAAATTTCAAAGTTAAACATGCGTCATAG242420.1495292468708621No Hit
CGTCATAGCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAA235060.14498945948958356No Hit
TGTGTCACCACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAAC233910.1442801177112588No Hit
CTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAA231850.14300947069965092No Hit
GGCAGGTTTCACCTTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACA223820.138056414630131No Hit
CCACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAA222730.13738408198806665No Hit
CCAAGCTGTGTCACCACGCTTTTAACTCAAATCATGTATAACTTTAATGG213130.13146262018639898No Hit
CCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAGT212700.13119738804319928No Hit
ATTTAATTGTCTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCA212440.1310370151194041No Hit
TTACAATTCAACATCGAGGTCGCAAACACTGTCGTTGATTAACTCTCAAA211910.13071010108243705No Hit
CTACATTATCACAGTCAGTGTTACCGCGGCCATTTAATTGTCTTTATTAG208100.1283600209299002No Hit
GGACGTTACAATTCAACATCGAGGTCGCAAACACTGTCGTTGATTAACTC205000.12644788222311168No Hit
GAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGT202610.1249736849620715No Hit
GAACTATTGATTTTTGATCCTTTCGTACGAAAAAACAACATATTTTTGTT202250.12475163014450895No Hit
TTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCA199630.12313556452780382No Hit
TGGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTT192480.11872530912343676No Hit
CTGGTTCCAAGCCTACATTTTTATTGTCTAAATTTAATTTTTTTTTAAAC191320.11800979915573524No Hit
ATCCTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAG186440.11499972273988751No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTGGGGG185750.11457411767289265TruSeq Adapter, Index 22 (97% over 37bp)
CCACAATCACCAGTTTTTGCTCACACAATTCATGATTAAATCACTTGGTT181380.11187861891525852No Hit
GTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAA180710.11146535022701712No Hit
GAGATATCGAAAACAATTTATTCTGCTACGAAATATATAATTATTTTCAG179480.11070666293367847No Hit
GCGCTGTTATCCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCAT179080.11045993535860896No Hit
GCCACAATCACCAGTTTTTGCTCACACAATTCATGATTAAATCACTTGGT178790.11028105786668359No Hit
GCTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTT171320.10567342040226092No Hit
GCTATTTCCAAGTTCGATTAGCCTTTCACCCCTAATCACAAATTTTCTGA163220.10067718700710385No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)