Bismark report for: ../../data/03-Peve-bismark/POR-74-TP3_R1_001.fastp-trim.fq.gz and ../../data/03-Peve-bismark/POR-74-TP3_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/srlab/sr320/github/timeseries_molecular/E-Peve/data/ with the specified options: -q --score-min L,0,-0.6 -p 10 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 5882 Mapping efficiency: 58.8% Sequence pairs with no alignments under any condition: 3233 Sequence pairs did not map uniquely: 885 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2908 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 2974 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 298733 Total methylated C's in CpG context: 3831 Total methylated C's in CHG context: 216 Total methylated C's in CHH context: 878 Total methylated C's in Unknown context: 6 Total unmethylated C's in CpG context: 44381 Total unmethylated C's in CHG context: 52615 Total unmethylated C's in CHH context: 196812 Total unmethylated C's in Unknown context: 389 C methylated in CpG context: 7.9% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in Unknown context (CN or CHN): 1.5% Bismark completed in 0d 0h 0m 16s