FastQCFastQC Report
Thu 20 Feb 2025
POR-83-TP1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-83-TP1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18220049
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA1897351.0413528525636786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1651740.9065508001652465No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG761750.4180833981291708No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT750500.4119088812549297No Hit
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG746040.40946102834300824No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT715150.39250717712120314No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG714870.39235350025677757No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT674830.37037770864392294No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA577550.31698597517492955No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC522270.28664577136976965No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC510820.2803614853066531No Hit
TGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGG469160.2574965632639078No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAG436480.2395602778016678No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC435810.23919255101893527No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG411530.22586657148946196No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG409480.22474143730348914No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC402380.22084463109841254No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA401820.2205372773695614No Hit
TCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATG372040.2041926451460147No Hit
CAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAAC349320.19172286528976953No Hit
CAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGG344000.18880300486568397No Hit
CTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAG328760.18043859267337864No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG309780.1700214966491034No Hit
CGGGAATGCAGCCCAAAAATGGTGGTAAACTCCATCTAAAGCTAAATACT302170.16584477901239453No Hit
TTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGC296490.16272733404833323No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA295860.16238156110337576No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA288320.15824326268277325No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG282340.15496116393539885No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT265760.14586129817762838No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA264890.14538380220602043No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA256610.14083935778657894No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT252810.1387537431979464No Hit
GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC250830.13766702822807997No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA250270.13735967449922884No Hit
GTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAA249900.13715660149980935No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA249500.13693706312205856No Hit
CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATA247630.1359107212060736No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA246310.13518624455949596No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT238150.13070766165337974No Hit
TAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACT234890.12891842387471075No Hit
CGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCG234110.1284903240380967No Hit
CGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTA233080.12792501271538842No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA229390.12589977118063733No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG226440.12428067564472522No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC219140.1202741002507732No Hit
CTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG213920.11740912442112532No Hit
TCTAGAGCTAATACATGCGAAAAGTCCCGACTTCTGGAAGGGATGTATTT212470.11661329780177868No Hit
CTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGCGGACCGCAAGGT210250.11539485980526178No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT207800.11405018724153816No Hit
TGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATT207020.1136220874049241No Hit
GCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGG205780.11294151843389663No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACC204170.1120578764634497No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAC204140.11204141108511838No Hit
CTTTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGA199800.10965941968652225No Hit
GTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTAT199660.10958258125430947No Hit
TTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAAC197330.10830377020391109No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACT194200.10658588239801112No Hit
CAAATTTCTGCCCTATCAACTGTCGATGGTAAGGTAGTGGCTTACCATGG191680.10520279061818111No Hit
CGAACAACGAACTTAGAACTGGCAGTGACTAGGGGAATCCGACTGTTTAA191400.10504911375375556No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG187010.10263968005794057No Hit
CCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAT186980.10262321467960926No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGA183010.10044429628043262No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA182580.10020829252435051No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)